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恒河猴同种异体原位肝移模型的改进(PDF)

《中国普外基础与临床杂志》[ISSN:1007-9424/CN:51-1505/R]

期数:
2010年07期
页码:
694-698
栏目:
移植工程
出版日期:
2010-07-22

文章信息/Info

Title:
 Improvements of Orthotopic Liver Transplantation Model in Rhesus Monkey
 
稿件编号:
201007015
作者:
冉江华; 张升宁; 刘静; 李铸; 李来邦; 吴淑媛; 李立
昆明市第一人民医院暨昆明医学院附属甘美医院肝胆胰一科、云南省器官移植研究所肝移植研究中心（昆明 650011）
Author(s):
 RAN Jiang-hua;  ZHANG Sheng-ning;  LIU Jing;  LI Zhu;  LI Lai-bang;  WU Shu-yuan;  LI Li.
 The First Department of Hepatobiliaropancreatic Surgery, The First People’s Hospital of Kunming City and The Affiliated Ganmei Hospital of Kunming Medical College, Liver Transplantation Center of Organ Transplantation Institute of Yunnan Province, Kunming 650011, China
关键词:
肝移植;  动物模型;  恒河猴;  灵长类动物;  血管重建
Keywords:
Liver transplantation;  Animal model;  Rhesus monkey;  Primates;  Vascular reconstruction
分类号:
R657.3
DOI:
-
文献标识码:
A
摘要:
目的  探讨恒河猴原位肝移植模型建立的最佳方案。
方法 选用10对健康恒河猴进行同种异体肝移植，借鉴各种动物模型建立的方法，使用门静脉袖套法建立稳定的恒河猴原位肝移植模型。
结果 10只恒河猴原位肝移植模型手术均成功。供体切取手术时间(20±5) min，供肝修整时间(30±7) min； 受体手术时间(180±35) min,受体无肝期(17±4) min。术后24 h存活者9只,1只术后9 h死于腹腔内出血; 72 h存活者8只，于术后38 h因消化道出血再死亡1只; 1周存活5只,3只分别于术后9、11和11 d死于排斥反应； 最长存活32 d者也死于排斥反应。所有受体均无门静脉血栓形成及胆道并发症发生。
结论 改进后的恒河猴同种异体原位肝移植模型具有操作简便、手术成功率高的优点, 是肝移植临床前期研究较理想的动物模型。
Abstract:
Objective     To explore improvement of orthotopic liver transplantation model in rhesus monkey.
Methods      Healthy rhesus monkeys were chosen to perform orthotopic liver transplantation for 10 cases. The model was established by drawing on a variety of animal model methods, and the portal vein cuff method was used to establish stable model of orthotopic liver transplantation in rhesus monkeys.
Results      Ten orthotopic liver transplantation models in rhesus were performed, and the achievement ratio of operation was 10/10. The time of donor hepatectomy and donor preparation was (20±5) min and (30±7) min, respectively. The operation time of recipient and anhepatic phase were (180±35) min and (17±4) min, respectively. After 24 h of operation 9 cases survived, one case died of intra-abdominal hemorrhage after 9 h of operation. After 72 h of operation 8 cases survived, and one case died of upper gastrointestinal bleeding after 38 h of operation. After one week of operation 5 cases survived, and 3 cases died of rejection after 9, 11, and 11 d of operation, respectively. The longest survival time was 32 d, but all of them also died of rejection. No portal vein thrombosis and biliary complications were found in all recipients.
 Conclusion    The improved rhesus monkey model of orthotopic liver transplantation is easy to perform with high achievement ratio of operation. It is an ideal animal model for pre-clinical studies of liver transplantation.

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备注/Memo

备注/Memo:
 【通讯作者】冉江华，E-mail: rjh2u@163.com
【作者简介】冉江华（1965年-），男，重庆市人，博士，教授，主要从事器官移植、免疫耐受、肝胆胰外科等方面的研究。
更新日期/Last Update: 2010-07-27